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Tag: Lab

  • The Training of Trainers on the Integrated Gene-Xpert Testing Program for Malawi at Dedza Pottery  Lodge

    The Training of Trainers on the Integrated Gene-Xpert Testing Program for Malawi at Dedza Pottery  Lodge

    The Public Health Institute of Malawi (PHIM), a leading institution in healthcare, through the Division of National Public Health Laboratory Services, with financial support from the World Bank, has organised a refresher training with a vital purpose of promoting integrated laboratory testing using the Gene-Xpert.

    The training of trainers took place at Dedza Pottery Lodge from the 13th –  17th of May, 2024. The meeting was opened by the Deputy Director of the Public Health Institute of Malawi (PHIM) responsible for the laboratory, Mr Joseph Bitilinyu Bangoh.

    IntegratedvGene-Xpert Trainees attentively listened to opening remarks by the Deputy Director of the Public Health Institute of Malawi (PHIM) responsible for the Laboratory, Mr Jospeh Bitilinyu Bangoh.

    GeneXpert is a PCR machine that amplifies DNA or RNA for viral particles and organisms. In Malawi, the machine was mainly used for the detection of Mycobacterium tuberculosis (MTB) and its associated resistance to Rifampicin and Isoniazid, HIV Viral Load (VL) and Early Infant Diagnostic (EID) and later, with the coming of SARS-CoV 2, the tests were added to the machine to detect viruses.

    It is of late that the machine, with the advancing technology, is now used to detect MTB in children’s stool, following the principle that children could shed MTB in their stool after swallowing the sputum at night. Another platform was added in the detection of Human papillomavirus (HPV) in women for early detection of the virus before advancing to advanced cervical cancer. 

    This detection of multiple human infections shall trigger the laboratory to have several integrated testing activities targeting GeneXPert.

    The trainees getting clarified on a point.
  • BIOSAFETY & BIOSECURITY TWG IN LILONGWE TO FINALISE NATIONAL GUIDELINES.

    BIOSAFETY & BIOSECURITY TWG IN LILONGWE TO FINALISE NATIONAL GUIDELINES.

    The first technical working group (TWG) for biosafety and biosecurity took place in Lilongwe on Nov 7 th and 8 th 2023. The purpose of the meeting was to finalise the national guidelines as well as to initiate a long-standing collaboration to safeguard workers and society from dangers of bio-hazards.

    As he opened the meeting, Mr Joseph Bitilinyu, the acting director of PHIM, commented that biosafety and biosecurity has a high priority, both for Malawi and for the African Union.

    The new TWG consist of prominent representatives from several sectors of society. Experts from both animal, human, agriculture and environmental health as well as from police, defence and academia were present. Experts from collaborating partners were also attending the TWG meeting.

    The ongoing outbreak of anthrax in neighbouring Zambia illustrates core questions of biosafety and biosecurity, as it is dangerous for animals and humans and can be used for bioterror. “Biosafety” elates to assuring that biological materials do not harm the persons handling them. “Biosecurity” relates to minimizing the risk that biological material comes astray to threaten the society. Risk assessment is the foundation of good biosafety and biosecurity and should answer three questions; What are the chances of the adverse advent? How severe would it be if it happened? What can we do to mitigate the risks?

    Through two full days, the current situation in Malawi and the rest of the world was presented, but emphasis was put on thorough discussion of each paragraph of the guidelines. It was agreed that Mr.Mabvuto Chiwaula and his team, who has lead this work so far, was trusted to incorporate all the comments into the draft guidelines before getting the final go-ahead.

    On closing the meeting, all members expressed their will to continue their contribution to the TWG. Several participants commented that both the meeting and the document is a milestone of its own, while other added that it is rare to come to such a truly multisectoral meeting. Another commented that “One Health is a song that we are all tuned into after this meeting”.

    Visiting experts from the Norwegian Institute of Public Health, PHIMs sister institute, did not go home empty handed. Head of department dr Siri Feruglio, commented that it was indeed very inspiring and impressive, and even touching, to see the constructive and nice way the members of different parts of society collaborate and contribute for the common good.

  • Quality of Malaria and Tuberculosis Genomic Sequencing in Malawi.

    Genomic Surveillance Webinar | PHIM Malawi
    Pathogen Genomic Surveillance

    Quality of Malaria and Tuberculosis
    Genomic Sequencing in Malawi

    Analyzing the quality and impact of Next-Generation Sequencing (NGS) for Malaria and Tuberculosis. Strengthening national diagnostic precision through high-fidelity genomic data.

    Date & Time

    Wed, 22 April | 2 PM – 3 PM

    Lead Scientists & Contributors

    Dr. Matthew Kagoli

    Dr. Matthew Kagoli

    Director, PHIM

    Dr. Kagoli provides comprehensive oversight of the strategic integration of genomic surveillance into Malawi’s National Health Security Framework. He focuses on ensuring that genomic data informs real-time public health responses and national policy. His leadership is pivotal in bridging the gap between laboratory research and community-level interventions. He is a strong advocate for evidence-based decision-making in the Malawian healthcare system.

    Dr. Bitilinyu Bango

    Dr. Bitilinyu Bango

    Deputy Director, PHIM

    Dr. Bango leads the technical implementation of advanced sequencing platforms specifically targeted at Malaria drug-resistance monitoring. He is instrumental in establishing TB lineage characterization protocols that meet international standards. His expertise ensures that the laboratory workflows remain efficient and scalable during public health emergencies. He actively collaborates with international partners to bring cutting-edge technology to the PHIM laboratories.

    Dr. Bernard Mvula

    Dr. Bernard Mvula

    Head of Knowledge Management

    Dr. Mvula is a specialist in bioinformatics and data architecture, focusing on the complex translation of raw sequencing reads into actionable insights. He manages the national genomic data repositories, ensuring data integrity and accessibility for research and surveillance. His work is essential for developing automated pipelines that reduce the turnaround time for diagnostic reporting. He also leads training initiatives to build bioinformatics capacity within the Malawian scientific community.

    Flora Dimba

    Flora Dimba

    Deputy Director, E&S Unit

    Ms. Dimba provides expert leadership in epidemiological field linkage, ensuring that genomic findings for TB and Malaria are geographically mapped. Her work allows for targeted interventions in hotspots where drug resistance or specific lineages are emerging. She coordinates with district health offices to synchronize sample collection with surveillance data. Her efforts are vital for maintaining the resilience and transparency of Malawi’s integrated health monitoring systems.

    Technical Delivery

    Deep dive into laboratory protocols, bioinformatic pipelines, and quality assurance for pathogen sequencing.

    • Sequencing Library Prep Standards
    • Bioinformatics Pipeline Review
    • QA/QC for Clinical Data

    Seminar Program

    Wednesday, 22nd April
    02:00 PM

    Opening & Policy Context

    State of Genomics in Malawi: Dr. Matthew Kagoli

    02:15 PM

    Malaria & TB Quality Assessment

    Benchmarking Sequencing Accuracy: Dr. Bitilinyu Bango

    02:35 PM

    Bio-Surveillance & Resistance Mapping

    Data Integration: Dr. Bernard Mvula & Flora Dimba

    02:50 PM

    Interactive Q&A Session

    Open Floor: Addressing Laboratory Challenges

    Webinar Registration

    Join the specialized session on Pathogen Genomic Quality.

    Research & Resources

    Authored & Published By

    Moses Nyambalo Phiri

    Public Health Institute of Malawi

    Ministry of Health, Republic of Malawi

    Advancing Diagnostic Excellence in Malawi